Journal: International Journal of Molecular Sciences

Article Title: The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes

doi: 10.3390/ijms23010073

Figure Lengend Snippet: ABCC3, CPS1, and TRIP6 expression in paclitaxel-resistant NCI/ADR-RES, SKOV-3/RES, and OVCAR-3/RES. ( A ) Bar graph showing relative expression of ABCC3, CPS1 and TRIP6 genes in paclitaxel-resistant ovarian cancer cell lines (technical triplicates). ( B ) Representative immunoblots of CPS1 in paclitaxel-resistant ovarian carcinoma cell lines. CPS1 silenced SKOV-3/RES cells or non-specific siRNA transfected SKOV-3/RES cells and human liver tissue were used as controls. ( C ) Representative immunoblot of ABCC3 in paclitaxel-resistant cell lines. ABCC3 silenced MCF-7/RES breast cancer cells or non-specific siRNA transfected MCF-7/PacR breast cancer cells and human liver tissue were used as controls. ( D ) Representative immunoblot of TRIP6 in paclitaxel-resistant ovarian carcinoma cell lines. β-ACTIN served as a loading control. The size of TRIP6 band was confirmed previously by us .

Article Snippet: Following primary antibodies were applied onto the membranes and incubated overnight at 4 °C: anti -TRIP6 (HPA052813) and anti -ACTIN (clone AC-40) (A3853) from Merck (Darmstadt, Germany), anti -CPS1 [EPR7493-3] (ab129076) from Abcam (Cambridge, UK) and anti-MRP3 (PA5-23653) from ThermoFisher.

Techniques: Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: The Role of TRIP6, ABCC3 and CPS1 Expression in Resistance of Ovarian Cancer to Taxanes

doi: 10.3390/ijms23010073

Figure Lengend Snippet: Significant differences in the mRNA levels of ( A ) CPS1 and ( B ) TRIP6 genes and ( C ) CPS1 and TRIP6 proteins in ovarian carcinoma mouse xenografts after the treatment with paclitaxel and novel SB-Ts in vivo. ( A , B ) Gene expression differences are shown as a mean of fold change (2 −∆∆CT ) ± SD, between the control group (Group I), group treated with 10 mg/kg paclitaxel (Group II), 9 mg/kg paclitaxel + 1 mg/kg SB-T-121605 (Group III), 7 mg/kg paclitaxel + 3 mg/kg SB-T-121605 (Group IV), 9 mg/kg paclitaxel + 1 mg/kg SB-T-121606 (Group V), and 7 mg/kg paclitaxel + 3 mg/kg SB-T-121606 (Group VI). Statistical analysis was performed by the two-tailed Student´s t -test * p < 0.05, ** p < 0.01, *** p < 0.001). ( C ) Representative immunoblot of CPS1, TRIP6, and β-ACTIN proteins in each group of mouse xenografts. Each group consisted of five mice.

Article Snippet: Following primary antibodies were applied onto the membranes and incubated overnight at 4 °C: anti -TRIP6 (HPA052813) and anti -ACTIN (clone AC-40) (A3853) from Merck (Darmstadt, Germany), anti -CPS1 [EPR7493-3] (ab129076) from Abcam (Cambridge, UK) and anti-MRP3 (PA5-23653) from ThermoFisher.

Techniques: In Vivo, Gene Expression, Control, Two Tailed Test, Western Blot